LC-MS use, but we are left with the 2.0

• Filter the solvent into a 5 mL glass amber vial using a 0.22 micron nylon syringe-filter, and then cap it. • Label the bottle with the sample ID. • Transfer 0.5 mL of extracted sample into a 2 mL HPLC vial and dilute it with 0.5 mL of LC/MS grade acetonitrile and mix it. LC Method and MS Source Conditions

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This confirms that there was an absence of elution components from the vial in random inspections using LC/MS/MS and GC/MS. Therefore, this product can be used with confidence, with no concern for ghost peaks originating from the vial.

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LC-MS Contamination Bill Mahn Page 2 8/11/2005 General Discussion Chemical contamination is one of the more common problems with LC/MS. I recommend using the highest purity chemicals available. • Try Burdick and Jackson solvents and water. These seem to be uniformly better than other suppliers.

interfere with LC-MS results 5 General system care, maintenance and laboratory practice 6 Sample preparation is crucial for minimizing contamination 7 Proper mobile phase preparation to minimize contaminants 17 Effects of column choice on LC-MS performance 24 Useful LC-MS resources 28 Appendix I. Common mass spectrometry

1.10 Use clean vials, caps, and plates a. Use Aijiren Technology-brand vials; they have been certified as contaminant-free.4 Other vials may not be clean or may have caps containing adhesives that can contaminate the sample manager. b. Make sure the liner on your vial and bottle caps does not contain contaminants (check the manufacturer’s description):

•Tune/calibrate LC and MS components •Monitor variability of metrics over several injections •Always use the same method for LC and MS, even if it is different than your sample method •Ask the vendor for performance specifications for both LC and MS parameters •Note when system performance deviates

There are many different manufacturers of LC and MS systems, each with their own particular traits and operating instructions, but generally they all follow the same principles. Hence, the guidance in this document should be relevant regardless of the particular instrumentation used in a laboratory. 1.2 Why Use LC-MS for quantitation?

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• Ultra-low protein binding Plastic Vials Provide: • Inert and free from clarifiers and antioxidants • 100 % virgin medical grade polypropylene • No contribution to adsorption, pH change, or baseline noise Phenomenex l WEB: www.phenomenex.com Strata Low-Bind 96-Well Collection Plate (Part No.: AH1-7036)

We have a full range of HPLC vials, GC vials, EPA vials, GC/MS vials, LC/MS vials, storage vials, syringe filters and filter membranes. We have more than 1000 products, and 20-30 NEW items every year thanks to our R & D team. Please ask for catalog. * 2.Are you a manufacturer for all your products?

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